Exosome RNA-seq – A Primer
Exosomes are a type of membranous vesicle derived from cells and released into the extracellular space. They are generally 30-100 nm in diameter, containing nucleic acid and protein, and can [...]
Ribosomal RNA removal is never a clean sweep
Ribosomal RNA (rRNA) constitutes a large majority (> 97%) of the RNA species in any total RNA preparation. Therefore, to uncover the complexity of the < 3% mRNA and non coding RNA in a [...]
ribosomal RNA Removal
Since the early days of RNA-seq there has been a heavy focus on the development of effective methods for the removal of ribosomal RNA (rRNA), which account for 95-98% of the transcriptome, [...]
The In-Between
The power of next generation sequencing lies in its ability to serve as a readout for a wide range of biological applications. Many of these applications such as whole genome sequencing, [...]
Sequencing of Low-Diversity Libraries
As next-generation sequencing applications increase, the likelihood that libraries will deviate from a perfectly balanced base composition is becoming unavoidable. Amplicon libraries, [...]
